A rapid microextraction by packed sorbent - liquid chromatography tandem mass spectrometry method for the determination of dexamethasone disodium phosphate and dexamethasone in aqueous humor of patients with uveitis.

Title A rapid microextraction by packed sorbent - liquid chromatography tandem mass spectrometry method for the determination of dexamethasone disodium phosphate and dexamethasone in aqueous humor of patients with uveitis.
Authors F. Bianchi; M. Mattarozzi; N. Riboni; P. Mora; S.A. Gandolfi; M. Careri
Journal J Pharm Biomed Anal
DOI 10.1016/j.jpba.2017.05.025
Abstract

A new method based on microextraction by packed sorbent (MEPS) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of dexamethasone and dexamethasone disodium phosphate in human aqueous humor. A central composite design was applied to investigate the effects of both loading and eluting cycles in the MEPS procedure; subsequently the multicriteria method of the desirability functions allowed to find the best conditions for the simultaneous extraction of both the analytes. Detection was performed on a LTQ XL linear ion trap mass spectrometer operating in the positive electrospray ionization mode applying multiple reaction monitoring mode. The assay was validated in accordance with the guidelines bioanalytical method validation obtaining quantitation limits in the low ?g/L range, a precision characterized by RSD?16% and recovery rates in the 91-119% range.

Citation F. Bianchi; M. Mattarozzi; N. Riboni; P. Mora; S.A. Gandolfi; M. Careri.A rapid microextraction by packed sorbent - liquid chromatography tandem mass spectrometry method for the determination of dexamethasone disodium phosphate and dexamethasone in aqueous humor of patients with uveitis.. J Pharm Biomed Anal. 2017;142:343347. doi:10.1016/j.jpba.2017.05.025

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Sodium

Sodium Bohr ModelSee more Sodium products. Sodium (atomic symbol: Na, atomic number: 11) is a Block D, Group 5, Period 4 element with an atomic weight of 22.989769. The number of electrons in each of Sodium's shells is [2, 8, 1] and its electron configuration is [Ne] 3s1. The sodium atom has a radius of 185.8 pm and a Van der Waals radius of 227 pm. Sodium was discovered and first isolated by Sir Humphrey Davy in 1807. In its elemental form, sodium has a silvery-white metallic appearance. It is the sixth most abundant element, making up 2.6 % of the earth's crust. Sodium does not occur in nature as a free element and must be extracted from its compounds (e.g., feldspars, sodalite, and rock salt). The name Sodium is thought to come from the Arabic word suda, meaning "headache" (due to sodium carbonate's headache-alleviating properties), and its elemental symbol Na comes from natrium, its Latin name.

Phosphorus

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