A "turn-off" fluorescent biosensor for the detection of mercury (II) based on graphite carbon nitride.

Title A "turn-off" fluorescent biosensor for the detection of mercury (II) based on graphite carbon nitride.
Authors J. Li; H. Wang; Z. Guo; Y. Wang; H. Ma; X. Ren; B. Du; Q. Wei
Journal Talanta
DOI 10.1016/j.talanta.2016.09.066
Abstract

A novel and simple fluorescent biosensor has been realized through the fluorescence quenching of graphite carbon nitride (g-C3N4) for mercuric ion (Hg(2+)) detection. In this assay, the g-C3N4 sheets which were functionalized with single-stranded DNA (ssDNA) aptamer showed strong fluorescence emission at 440nm under the excitation of 380nm in the absence of Hg(2+). When added to the assay solution, Hg(2+) was embedded in the hairpin-shaped double-stranded DNA (dsDNA) due to the formation of the thymine-Hg(2+)-thymine (T-Hg(2+)-T) complex, which made the Hg(2+) close to the surface of g-C3N4 sheet. Therefore, the fluorescence of g-C3N4 was quenched. This sensor has good selectivity with a limit of detection as low as 0.17nM under optimal conditions. The present work demonstrates that the g-C3N4-based fluorescent sensor has a promising application for detection of metal ions in real samples.

Citation J. Li; H. Wang; Z. Guo; Y. Wang; H. Ma; X. Ren; B. Du; Q. Wei.A "turn-off" fluorescent biosensor for the detection of mercury (II) based on graphite carbon nitride.. Talanta. 2017;162:4651. doi:10.1016/j.talanta.2016.09.066

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6 C 12.010700000 Carbon

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