Amperometric flow injection analysis of glucose using immobilized glucose oxidase on nano-composite carbon nanotubes-platinum nanoparticles carbon paste electrode.

Title Amperometric flow injection analysis of glucose using immobilized glucose oxidase on nano-composite carbon nanotubes-platinum nanoparticles carbon paste electrode.
Authors M. Amatatongchai; W. Sroysee; S. Chairam; D. Nacapricha
Journal Talanta
DOI 10.1016/j.talanta.2015.11.072
Abstract

We report a novel amperometric glucose biosensor based on glucose oxidase (GOx) immobilized on a carbon nanotube (CNTs)-poly(diallyldimethyl-ammonium chloride) (PDDA)-platinum nanoparticle (PtNPs) modified carbon-paste electrode (CNTs-PDDA-PtNPs/CPE). The CNTs-PDDA-PtNPs composite materials were characterized by TEM and electrochemical techniques. Cyclic voltammetric results reveal direct electron transfer of the immobilized GOx, indicated by two quasi-reversible redox peaks at a potential of 0.37V (vs. Ag/AgCl) in phosphate buffered solution (PBS) (0.10M, pH 7). The biosensor provides good glucose oxidation activity and retention of GOx electrocatalytic activity due to CNTs-PDDA-PtNPs enhancement of the redox response. The carbon paste electrode was installed as working electrode in a flow through electrochemical cell of a flow injection (FI) system. Glucose was quantified using amperometric measurements at 0.5V vs. Ag/AgCl and PBS carrier (0.10M, pH 7.0) at a flow rate of 1.0mLmin(-1). The linear working ranges for glucose measurements were 0.1-3mM (r(2)=0.995) and 5-100mM (r(2)=0.997), with corresponding sensitivities of 0.127 and 0.060 (?As) mM(-1), respectively. The system provides good precision of 2.8% R.S.D with a calculated detection limit (3S/N) of 15?M. The proposed method was successfully applied to determination of glucose in food and pharmaceutical samples with throughput of 200 samplesh(-1).

Citation M. Amatatongchai; W. Sroysee; S. Chairam; D. Nacapricha.Amperometric flow injection analysis of glucose using immobilized glucose oxidase on nano-composite carbon nanotubes-platinum nanoparticles carbon paste electrode.. Talanta. 2017;166:420427. doi:10.1016/j.talanta.2015.11.072

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Platinum

See more Platinum products. Platinum (atomic symbol: Pt, atomic number: 78) is a Block D, Group 10, Period 6 element with an atomic weight of 195.084. The number of electrons in each of platinum's shells is [2, 8, 18, 32, 17, 1] and its electron configuration is [Xe] 4f14 5d9 6s1. The platinum atom has a radius of 139 pm and a Van der Waals radius of 175 pm. Platinum Bohr ModelElemental PlatinumPlatinum was discovered and first isolated by Antonio de Ulloa in 1735. It is one of the rarest elements in the earth's crust, occurring at a concentration of only 0.005 ppm. Platinum is found uncombined as a free element and alloyed with iridium as platiniridium. In its elemental form, platinum has a grayish white appearance. It is highly resistant to corrosion: the metal does not oxidize in air at any temperature. It is generally non-reactive, even at high temperatures. The origin of the name "platinum" comes from the Spanish word platina, meaning silver.

Carbon

See more Carbon products. Carbon (atomic symbol: C, atomic number: 6) is a Block P, Group 14, Period 2 element. Carbon Bohr ModelThe number of electrons in each of Carbon's shells is 2, 4 and its electron configuration is [He]2s2 2p2. In its elemental form, carbon can take various physical forms (known as allotropes) based on the type of bonds between carbon atoms; the most well known allotropes are diamond, graphite, amorphous carbon, glassy carbon, and nanostructured forms such as carbon nanotubes, fullerenes, and nanofibers . Carbon is at the same time one of the softest (as graphite) and hardest (as diamond) materials found in nature. It is the 15th most abundant element in the Earth's crust, and the fourth most abundant element (by mass) in the universe after hydrogen, helium, and oxygen. Carbon was discovered by the Egyptians and Sumerians circa 3750 BC. It was first recognized as an element by Antoine Lavoisier in 1789.

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