An ultrasensitive electrochemiluminescent immunosensor based on graphene oxide coupled graphite-like carbon nitride and multiwalled carbon nanotubes-gold for the detection of diclofenac.

Title An ultrasensitive electrochemiluminescent immunosensor based on graphene oxide coupled graphite-like carbon nitride and multiwalled carbon nanotubes-gold for the detection of diclofenac.
Authors L. Hu; J. Zheng; K. Zhao; A. Deng; J. Li
Journal Biosens Bioelectron
DOI 10.1016/j.bios.2017.10.043
Abstract

In this study, a novel competition-type electrochemiluminescent (ECL) immunosensor for detecting diclofenac (DCF) was fabricated with graphene oxide coupled graphite-like carbon nitride (GO-g-C3N4) as signal probe for the first time. The ECL intensity of carboxylated g-C3N4 was significantly enhanced after being combined with graphene oxide (GO) which exhibited excellent charge-transport property. The sensing platform was constructed by multiwalled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs), which not only provided an effective matrix for immobilizing a large amount of coating antigen but also facilitated the electronic transmission rate to enhance the ECL intensity. Based on the synergistic effect of GO-g-C3N4 and MWCNTs-AuNPs composite, the proposed sensor showed high sensitivity, good stability, and wide linearity for the detection of DCF in the range of 0.005-1000ngmL-1 with a detection limit of 1.7pgmL-1. Furthermore, the developed immunoassay has been applied to real samples with satisfactory results. Therefore, this work provided a promising method for the detection of DCF and other small molecular compounds in the future.

Citation L. Hu; J. Zheng; K. Zhao; A. Deng; J. Li.An ultrasensitive electrochemiluminescent immunosensor based on graphene oxide coupled graphite-like carbon nitride and multiwalled carbon nanotubes-gold for the detection of diclofenac.. Biosens Bioelectron. 2018;101:260267. doi:10.1016/j.bios.2017.10.043

Related Elements

Carbon

See more Carbon products. Carbon (atomic symbol: C, atomic number: 6) is a Block P, Group 14, Period 2 element. Carbon Bohr ModelThe number of electrons in each of Carbon's shells is 2, 4 and its electron configuration is [He]2s2 2p2. In its elemental form, carbon can take various physical forms (known as allotropes) based on the type of bonds between carbon atoms; the most well known allotropes are diamond, graphite, amorphous carbon, glassy carbon, and nanostructured forms such as carbon nanotubes, fullerenes, and nanofibers . Carbon is at the same time one of the softest (as graphite) and hardest (as diamond) materials found in nature. It is the 15th most abundant element in the Earth's crust, and the fourth most abundant element (by mass) in the universe after hydrogen, helium, and oxygen. Carbon was discovered by the Egyptians and Sumerians circa 3750 BC. It was first recognized as an element by Antoine Lavoisier in 1789.

Gold

See more Gold products. Gold (atomic symbol: Au, atomic number: 79) is a Block D, Group 11, Period 6 element with an atomic weight of 196.966569. The number of electrons in each of Gold's shells is 2, 8, 18, 32, 18, 1 and its electron configuration is [Xe]4f142 5d10 6s1. Gold Bohr ModelThe gold atom has a radius of 144 pm and a Van der Waals radius of 217 pm. Gold was first discovered by Early Man prior to 6000 B.C. In its elemental form, gold has a metallic yellow appearance. Gold is a soft metal and is usually alloyed to give it more strength.Elemental Gold It is a good conductor of heat and electricity, and is unaffected by air and most reagents. It is one of the least reactive chemical elements. Gold is often found as a free element and with silver as a gold-silver alloy. Less commonly, it is found in minerals as gold compounds, usually with tellurium.

Nitrogen

See more Nitrogen products. Nitrogen is a Block P, Group 15, Period 2 element. Its electron configuration is [He]2s22p3. Nitrogen is an odorless, tasteless, colorless and mostly inert gas. It is the seventh most abundant element in the universe and it constitutes 78.09% (by volume) of Earth's atmosphere. Nitrogen was discovered by Daniel Rutherford in 1772.

Related Forms & Applications