Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles.

Title Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles.
Authors S.J. Yeo; D.Tuan Bao; G.E. Seo; C.Thi Bui; D.Thi Hoang Kim; N.Thi Viet Anh; T.Thi Thuy Tien; N.Thi Phuong Linh; H.J. Sohn; C.K. Chong; H.J. Shin; H. Park
Journal Sci Rep
DOI 10.1038/s41598-017-08328-9
Abstract

The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle-based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.

Citation S.J. Yeo; D.Tuan Bao; G.E. Seo; C.Thi Bui; D.Thi Hoang Kim; N.Thi Viet Anh; T.Thi Thuy Tien; N.Thi Phuong Linh; H.J. Sohn; C.K. Chong; H.J. Shin; H. Park.Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles.. Sci Rep. 2017;7(1):7933. doi:10.1038/s41598-017-08328-9

Related Elements

Europium

63 Eu 151.964000000 Europium

See more Europium products. Europium (atomic symbol: Eu, atomic number: 63) is a Block F, Group 3, Period 6 element with an atomic radius of 151.964. Europium Bohr ModelThe number of electrons in each of Europium's shells is 2, 8, 18, 25, 8, 2 and its electron configuration is [Xe]4f7 6s2. The europium atom has an atomic radius of 180 pm and a Van der Waals radius of 233 pm. Europium was discovered by Eugène-Anatole Demarçay in 1896, however, he did not isolate it until 1901. Europium was named after the continent of Europe.Elemental Europium Picture Europium is a member of the lanthanide or rare earth series of metals. In its elemental form, it has a silvery-white appearance but it is rarely found without oxide discoloration. Europium is found in many minerals including bastnasite, monazite, xenotime and loparite. It is not found in nature as a free element.

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