Reduced graphene oxide and gold nanoparticle composite-based solid-phase extraction coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry for the determination of 9 mycotoxins in milk.

Title Reduced graphene oxide and gold nanoparticle composite-based solid-phase extraction coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry for the determination of 9 mycotoxins in milk.
Authors K. Jiang; Q. Huang; K. Fan; L. Wu; D. Nie; W. Guo; Y. Wu; Z. Han
Journal Food Chem
DOI 10.1016/j.foodchem.2018.05.041
Abstract

A reliable solid-phase extraction (SPE) procedure using reduced graphene oxide and gold nanoparticle (rGO/Au) composite as sorbent was proposed for purification and enrichment of aflatoxin B (AFB), aflatoxin M (AFM), ochratoxin A (OTA), zearalenone (ZEA), ?-zearalenol (?-ZOL), ?-zearalenol (?-ZOL), zearalanone (ZAN), ?-zearalanol (?-ZAL) and ?-zearalanol (?-ZAL) in milk. Main parameters affecting the performance of SPE procedure were thoroughly investigated. The optimized conditions included 2% acetonitrile in water as the loading solution, 5% methanol in water as the washing solution, and methanol/acetonitrile/formic acid (50/49/1, v/v/v) as the elution solvent. Satisfactory linearity (R???0.992), high sensitivity (limit of quantification in the range of 0.02-0.18?ng?mL), adequate recovery (70.2-111.2%), and acceptable precision (RSD, 2.0-14.9%) were obtained when the optimal sample pretreatment protocol was combined with ultra-high-performance liquid chromatography-tandem mass spectrometry detection. The applicability of the validated method was further verified in real milk samples.

Citation K. Jiang; Q. Huang; K. Fan; L. Wu; D. Nie; W. Guo; Y. Wu; Z. Han.Reduced graphene oxide and gold nanoparticle composite-based solid-phase extraction coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry for the determination of 9 mycotoxins in milk.. Food Chem. 2018;264:218225. doi:10.1016/j.foodchem.2018.05.041

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