Tetra-nickel substituted polyoxotungsate as an efficient sorbent for the isolation of His6-tagged proteins from cell lysate.

Title Tetra-nickel substituted polyoxotungsate as an efficient sorbent for the isolation of His6-tagged proteins from cell lysate.
Authors M.M. Wang; Q. Chen; D.D. Zhang; X.W. Chen; M.L. Chen
Journal Talanta
DOI 10.1016/j.talanta.2017.04.079
Abstract

By virtue of the flexible structure of polyoxometalates, Ni(2+) is encapsulated into trivacant lacunary tungstophosphate ligands by the form of [Ni4] cluster to offer a tetra-nickel substituted polyoxotungsate K6Na4[Ni4(H2O)2(PW9O34)2] (Ni4P2). The Ni4P2 is then immobilized onto the surface of SiO2 nanoparticles by self-assembly under electrostatic interactions to give the product of Ni4P2@SiO2 composites. Due to the specific affinity between substituted Ni(2+) in the polyoxotungsate and the histidine residues of protein, Ni4P2@SiO2 composites exhibit highly adsorption selectivity towards histidine protein. This Ni4P2@SiO2 composite is of high stability, and SDS-PAGE assay indicates that it can be used repeatedly as an efficient sorbent for the isolation of His6-tagged proteins from cell lysate with improved performance when compared with commercial NTA-Ni(2+) column.

Citation M.M. Wang; Q. Chen; D.D. Zhang; X.W. Chen; M.L. Chen.Tetra-nickel substituted polyoxotungsate as an efficient sorbent for the isolation of His6-tagged proteins from cell lysate.. Talanta. 2017;171:173178. doi:10.1016/j.talanta.2017.04.079

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Tungsten

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